双歧杆菌22-5胞外多糖(EPS)的分离、纯化及纯度鉴定  

Isolation and Purification of Exopolysaccharides Produced by Bifidobacterium sp 22-5

作　　者：欧阳清波[1] 李平兰[1] 李伟欣[1] 孙成虎[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院

出　　处：《食品与发酵工业》2005年第6期127-130,共4页Food and Fermentation Industries

基　　金：国家863资助项目(2002AA248041)

摘　　要：为了得到双歧杆菌22-5胞外多糖(EPS)的纯品,对EPS分离纯化方法进行了摸索,得到EPS产量较高的分离条件:发酵上清液中加入3倍体积95%冷乙醇,-20℃沉淀12h以上,8000r/min离心4次,热水溶解后用Sevag法去蛋白6次,通过SephoroseCL-6B凝胶过滤得到的吸收峰,经紫外光谱扫描和HPLC分析,鉴定为单一多糖组分。The isolation process of exopolysaccharides was presented and optimized in this paper to obtain pure EPS produced by Bifidobacterium sp 22-5. The optimum conditions were: remove cells and precipitated proteins by centrifugation for 10min at 8 000r/min for 4 times, precipitate the EPSs by adding 3 times the volume of cold 95% ethanol and remove proteins using Sevag method. The crude EPSs were then filtrated by Sephorose CL-6B. The peak was detected by HPLC and UV Spectrum.

关 键 词：胞外多糖 双歧杆菌 纯度鉴定 分离纯化方法 Sevag法 HPLC分析 CL-6B 分离条件 凝胶过滤 紫外光谱 多糖组分 EPS 上清液 20℃ 水溶解 吸收峰 乙醇 

分 类 号：Q936[生物学—微生物学]