水母雪莲细胞悬浮培养合成黄酮及抗氧化活性(英文)  

Flavonoid Biosynthesis and Antioxidant Activities in the Suspension Culture of Saussurea medusa Cells

作　　者：刘永刚[1,2] 高敏[1,3] 崔建云[4] 刘春朝[1,3] 

机构地区：[1]中国科学院过程工程研究所生化工程国家重点实验室 [2]中国农业大学食品科学与营养工程学院,北京100083 [3]中国科学院研究生院,北京100093 [4]中国农业大学食品科学与营养工程学院

出　　处：《西北植物学报》2005年第7期1421-1427,共7页Acta Botanica Boreali-Occidentalia Sinica

摘　　要：对水母雪莲细胞悬浮培养过程中细胞生长、黄酮积累和底物消耗的动力学过程进行了研究。经15d液体培养可获得最大生物量干重和黄酮产量分别为17.2g·L-1和607.8mg·L-1,通过调控基本培养基种类和有机添加物可提高雪莲细胞的生长和黄酮积累。获得的水母雪莲细胞培养物具有明显的抗氧化活性,其抗氧化活性与雪莲细胞中的黄酮含量相关。The kinetic processes of cellular growth,flavonoid accumulation and substrate consumption in the suspension culture of Saussurea medusa cells were studied,which showed that the highest dry biomass and flavonoid yield occurred after 15-day culture amounting to 17.2 g·L^(-1) and 607.8 g·L^(-1),respectively,and the adjustment of the medium kinds and the addition of organic additives could increase the growth and flavonoid accumulation of Saussurea medusa cells.In the culture of Saussurea medusa cells,the antioxidant activities appeared very remarkable and related to flavonoid yield in Saussurea medusa cells.

关 键 词：水母雪莲 黄酮 悬浮细胞 抗氧化活性 

分 类 号：Q946.8[生物学—植物学]