啤酒废酵母超氧化物歧化酶（SOD）的分离纯化及性质研究  

Purification and Partial Properties of Cupper/Zine Superoxide Dimutase from Yeast(Saccharomyces Cerevisiae)

作　　者：曾仕廉[1] 张宏[1] 

机构地区：[1]安徽大学生物系,北京肿瘤研究所

出　　处：《安徽大学学报（自然科学版）》1995年第2期88-92,共5页Journal of Anhui University(Natural Science Edition)

摘　　要：啤酒废酵母（１０８－１０９细胞／ｍｌ）通气发酵后，经乙醇－氯仿沉淀，加热，调ＰＨ，（ＮＨ４）２ＳＯ４沉淀等除去杂蛋白质。最后经ＳｅｐｈａｄｅｘＧ－７５层析得到纯化的ＣｕＺｎ－ＳＯＤ，酶比活在１５００单位／ｍｇ蛋白以上，酶的分子量为１４８０００，由四个亚基组成，紫外最大吸收峰位于２５６ｕｍ处。Ａ２５６．４／２８０＝１．２２．After fermentation by air of waste saccharomyces cerevisiae, predpitation of contaminat proteins with chloroform/ethanol, heat denataration, (NH4)2 SO4 salting out and control of PH value, the predpitated proteins were separated and the SOD contuining solution was purified by gel filtration (sephadex G-75). Said process to give a field of SOD with an activity of 1500 nits/mg, the molecular weight of Cu. Zn--SOD from yeast was 148000 consisted of four Subunits, the enzyme exhibited one absorption maximuxn in the Ultraviolet at 256. 4um, A 256. 4/A 280=1. 22.

关 键 词：超氧化物歧化酶 啤酒 废酵母 废物综合利用 

分 类 号：X792.05[环境科学与工程—环境工程]