海洋细菌产琼胶酶的条件优化  

Optimization Growth Conditions of an Agarase-producing Marine Bacterium Sphingobacterium multivorumd

作　　者：陈惠源[1] 蔡俊鹏[1] 刘江涛[1] 

机构地区：[1]华南理工大学食品与生物工程学院,广东广州510640

出　　处：《现代食品科技》2005年第3期48-50,共3页Modern Food Science and Technology

摘　　要：研究了一株高产琼胶酶的海洋细菌多食鞘氨醇杆菌(Sphingobacteriummultivorumd)的最佳产酶条件,主要包括氮源、碳源、起始pH、温度、盐浓度和添加物等对产酶的影响,试验确定的最佳培养基组成为:NaCl2.0%;蛋白胨0.8%;MgS04·7H2O0.5%;KCl0.1;FeS04·7H2O0.002%;CaCl20.02%;NaH2PO40.06%;琼胶0.3%。该菌株产酶的最佳发酵条件为20℃,起始pH7.0下培养15h。经培养条件的优化后,菌株产酶活力高达18.4U/mL,较优化前提高了14倍,为酶的大量生产和活性琼胶低聚糖的酶法制备创造了条件。Sphingobacterium multivorumd which produces agarase was isolated. The optimum culture medium had been obtained with NaCl 2.0%, peptone 0.8%, MgSO4·7H2O 0.5%, KCl 0.1, FeSO4·7H2O 0.002%, CaCl2 0.02%, NaH2PO4 0.06% and 0.3% of agar, pH 7.0. After cultivated in 50mL medium in 100mL shake flask, 20℃ for 15h, the activity reached the highest value of 18.4U/mL which was 14 times higher than that under the original conditions. All of these lay a solid foundation for the large scale production of the enzyme and the development of bioactive Agaro-oligosaccharide by enzyme method.

关 键 词：琼胶酶 最佳产酶条件 Sphingobacterium multivorumd 

分 类 号：TQ925[轻工技术与工程—发酵工程]