乳铁蛋白的分离工艺研究  

Study on separating process of Lactoferrin

作　　者：吕立获[1] 周晓云[1] 姚婷婷[1] 芦国营[1] 吴绵斌[1] 

机构地区：[1]浙江工业大学,浙江杭州310032

出　　处：《中国乳品工业》2005年第8期10-13,共4页China Dairy Industry

摘　　要：讨论了从牛初乳中分离乳铁蛋白的工艺。结果表明,牛初乳经过离心脱脂得到脱脂乳,加入带羧甲基的弱酸性阳离子交换剂CM-Sepharose Fast Flow在4℃下进行动态吸附,用去离子水将没有吸附上的杂蛋白冲洗掉,然后使用质量分数为1.5%和5%的NaCl溶液进行阶跃洗脱;经过两步阶跃洗脱得到的乳铁蛋白纯度可达95%以上。The Lactoferrin is the living beings active material in the mammal mamma , and the original mainly talked about the technology of Lactoferrin departs from bovine colostrum ： The bovine colostrum obtains the skimmed milk after being at odds with the community to degrease , and accedes to brining Qiang＇s methyl weak acid cation exchange dose a CM-Sepharose Fast Flow being living to carry on static state below 4℃s adsorbing ; In the way of leave ion water shall not there is , and sundry albumen on the sticking rinses , afterwards employ the NaCl＇s liquor of 1.5% and 5% to carry on the stairs and jumps to wash comeing off ; After two jumps wash to come off over the Lactoferrin pureness attainability 95% of mamma that obtains.

关 键 词：牛初乳 乳铁蛋白 离子交换层析 

分 类 号：TQ936[化学工程]