2-脱氧葡萄糖对酵母虾青素积累及酶法提取的影响(英文)  

Effect of 2-deoxy-D-glucose on growth of Phaffia rhodozyma, astaxanthin content and extraction of astaxanthin

作　　者：蹇华丽[1] 朱明军[1] 吴振强[1] 梁世中[1] 

机构地区：[1]华南理工大学生物科学与工程学院,广州510640

出　　处：《贵州大学学报（自然科学版）》2005年第3期301-304,共4页Journal of Guizhou University:Natural Sciences

基　　金：广东省科技计划项目(2002C1040101);广州市科技计划项目(2003Z2-E0131)

摘　　要：2-脱氧葡萄糖是一种酵母细胞壁合成抑制剂,红发夫酵母培养中添加0.0005%-0.002%(w/v)的2-脱氧葡萄糖可使生物量基本保持不变,但能促进虾青素积累,在增加虾青素产量的同时缩短酶法提取的时间。当添加浓度为0.002%时,虾青素含量可提高到对照组的113.7%,若采用胞壁溶解酶对红发夫酵母破壁提取虾青素,作用12h提取率可达94.7%。同时,2-脱氧葡萄糖应在红发夫酵母培养的前36h添加。The concentration of 2-deoxy-D-glucose in YM medium significantly affected the biomass and astaxanthin content of Phaffia rhodozyma and the extraction of astaxanthin with lytic enzyme. Optimal concentration which led to 13.7% enhancement of astaxanthin content and, more importantly, made extraction of astaxanthin easy, is 0.002% （w/v）. When the yeast was cultivated under this condition, extractability of astaxanthin can reach 94.7% after 12h treatment with lytic enzyme. In addition, 2-deoxy-D-glucose should be added during the previous 36h of culture time of Phaffia rhodozyma.

关 键 词：2-脱氧葡萄糖 红发夫酵母 虾青素 胞壁溶解酶 

分 类 号：Q599[生物学—生物化学]