十五碳二元酸的发酵研究  

STUDIES ON MICROBIAL PRODUCTION OF TRIDECANE 1,13-DICARBOXYLIC ACID (DC_15) FROM n-PENTADECANE(nC_15)

作　　者：陈远童[1] 郝秀珍[1] 庞月川[1] 

机构地区：[1]中国科学院微生物研究所,北京100080

出　　处：《微生物学报》1995年第6期433-437,共5页Acta Microbiologica Sinica

基　　金：国家"八.五"攻关项目

摘　　要：以热带假丝酵母(candida tropicalis)T_(25-14)为出发菌株,经过紫外线和亚硝酸的诱变和筛选,得到一株从正十五烷(nC_(15)发酵生产十五碳二元酸(DC_(15)的优良突变株NP-6-126。摇瓶发酵4d,T_(25-14)产DC_(15)为35.8g/L,而NP-6-126则达到73.5g/L,产酸水平提高一倍以上。气相色谱分析表明,DC_(15)的纯度也从69％提高到94％。加入适量青霉素,对DC_(15)的产生有明显的促进作用。A mutant strain Candida tropicalis NP-6-126 producing pentadecane-dioic acid (DC15) from pentadecane (nC15) was obtained by mutagensis of UV radiation and sodium nitrite treatment on Candida tropicalis T25-14. The yield of DC15 and purity was 35.8g/L and 69% of starting strain T25-14 and 73.5g/L and 94% of mutant strain NP-6-126 for 4 days on shaking flask testing respectively.

关 键 词：十五碳二元酸 热带假丝酵母 发酵 

分 类 号：TQ921.7[轻工技术与工程—发酵工程]