高产复合酶菌株HD-1选育的研究  

STUDIES ON THE SCREENING OF HIGHLY PRODUCTIVE COMPOUND ENZYMES STRAIN HD-1

作　　者：赵小立[1] 李永泉[1] 贺筱蓉[1] 周红军[1] 翁醒华[1] 钱育军 泮伯良 

机构地区：[1]杭州大学生命科学学院,杭州310028 [2]浙江大学物理学系光学研究所,杭州310027

出　　处：《真菌学报》1995年第4期289-295,共7页

基　　金：浙江省教委基金课题

摘　　要：从产果胶酶的黑曲霉(Aspergillus niger)菌中分离出一支产酸性蛋白酶等多种水解酶的菌株No.3号。经铜蒸汽激光诱变,选育出一支高产复合酶的菌株HD-1,该菌在简单的固体发酵培养基上,28℃培养48hr,每克鲜曲的酶活力(U)为:酸性蛋白酶7500U、纤维素C_X酶9061U、纤维素C_L酶3581U、果胶酶5471U、糖化酶5582U。酶活平均提高率为38.86％,最高幅度是78.6％。该菌经多次连续传代和贮存一年后,产酶性状稳定。该菌株是目前国内饲料用酶制剂生产株中,酶系最齐全,产酶水平位于前列的优良菌株。No. 3 strain was screened from pectinase-producing strain of Asper-gtllus niger. This strain produces acid proteinase and a number of different hydro-lytic enzymes. The mutant strain HD-1 was selected from the parent strain No. 3 by irradiation of copper vapor laser. By incubation of HD-1 in a simple solid fermentation medium at 28℃ for 48hr, the enzyme activities were: acid proteinase 7500U, cellu-lase Cx 9061U, cellulase CL 3581U, pectinase 5471U and glucoamylase 5532U. The enzyme activity was increased by 38.86% in average and the maximum is 78.6%. The HD-1 strain produces five kinds of hydrolases with high enzyme activity. Its high capacity for producing enzymes remained stable after 20 times of subculture and one year of storage. In short, it was an ideal strain for the enzyme additives industry.

关 键 词：激光诱变 复合酶 黑曲霉 发酵 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]