保健油脂中多不饱和脂肪酸的GC/MS分析  

Analysis of the multi-unsaturated fatty acids in healthy oils by GC/MS

作　　者：付松[1] 徐先顺[1] 向奋飞[1] 

机构地区：[1]四川省疾病预防控制中心,成都610031

出　　处：《中国卫生检验杂志》2005年第9期1042-1044,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立保健油脂中多不饱和脂肪酸及角鲨烯的GC/MS分析方法.方法:多不饱和脂肪酸以KOH-甲醇法进行甲酯化,用异辛烷提取多不饱和脂肪酸甲酯,经HP-FFAP石英毛细管柱分离,用气相色谱-质谱联用仪(GC-MS)进行定性﹑定量分析.结果:5种多不饱和脂肪酸及角鲨烯的标准溶液浓度在 0.025～0.40 mg/ml 范围内线性关系良好,相关系数 r >0.9990;加标平均回收率范围为 94.9%～101%;相对标准偏差(RSD)<5%.结论:本方法简便快速,准确可靠,适用于保健油脂中多不饱和脂肪酸及角鲨烯的检测.Objective ：To develop a method for analyzing the multi-unsaturated fatty acids in healthy oils by GC/MS. Meth- ods： The multi - unsaturated fatty acids was fist methylated with potassium hydroxide - methanol （ KOH - MeOH）, extracted by iso-octane, isolated by HP - FFAP silica capillary column, identified and determined by gas chromatograph equipped with mass spectrometer. Results：The calibration curves were linear within the ranges of 0. 025 - 0. 40 mg/ml for all standard solution, relative coefficient was over 0. 9990;The average recoveries ranged from 94.9% to 101% ; Precisions （RSD） were under 5% for all the analyzed components. Condusion：The method was simple, rapid, accurate, and suitable for the determination of mulit - unsaturated fatty acids in healthy oils.

关 键 词：保健油脂 GC/MS分析 多不饱和脂肪酸 角鲨烯 

分 类 号：R155.5[医药卫生—营养与食品卫生学]