食品中转基因成分的PCR快速检测研究  

Study of rapid detection using PCR on genetically modified f ood

作　　者：冯家望[1] 吴小伦[1] 王小玉[1] 黄云君[1] 薄清如[1] 陈静静[1] 

机构地区：[1]珠海出入境检验检疫局,广东珠海519015

出　　处：《中国食品卫生杂志》2005年第5期412-415,共4页Chinese Journal of Food Hygiene

基　　金：珠海市科技攻关项目(2002-32)~~

摘　　要：为建立食品中转基因成分的快速检测方法,针对转基因大豆、玉米、油菜的多个相对稳定的转基因元件,包括35S、NOS、EPSPS、Cry1A、NPTⅡ等基因,同时选定植物本身固有的基因:大豆Lectin、玉米IVR、油菜Napin基因作为内源参照指示基因,设计、筛选出9对引物分别组成多重PCR,结合高灵敏度的聚丙烯酰胺凝胶电泳组成快速检测体系对转基因食品进行检测,1～2 d能完成整个检测过程.经测试:该检测体系稳定可靠、灵敏准确、特异性好,且操作简便、成本低廉,是一种进行转基因食品检测的良好技术模式.According to the stable in the genetical modified soybeen, corn, cole specific genes （35S, NOS, EPSPS, Cry1A, NPT Ⅱ ）, and the inherent control genes in plants： soybeen Lectin, corn IVR, cole Napin, nine primer pairs for multiplex-PCR were designed and screened out. Combining with the high sensitive polyactylamide gel eletrophoresis （PAGE）, we developed a rapid detection system for the transgenic food, which will take only 1 -2 d for the total detection and was proved to be a good technology model with simple operation, low cost and good credibility, accuration, and specificity.

关 键 词：食品 转基因 聚合酶链反应 食品分析 

分 类 号：TS207[轻工技术与工程—食品科学]