高强脉冲电场对脂肪酶的影响研究  

Study on the inactivation of lipase by the treatment of pulsed electric fields

作　　者：徐娅莉[1] 曾新安[1] 于淑娟[1] 

机构地区：[1]华南理工大学轻工与食品学院,广州五山510640

出　　处：《中国乳品工业》2005年第9期44-46,共3页China Dairy Industry

摘　　要：采用高强脉冲电场对脂肪酶进行处理,研究了电场强度、脉冲数目、温度、介质环境等因素对酶活的影响。结果表明,高强脉冲电场可引起脂肪的失活,且失活率与电场强度和脉冲数目呈正比,但酶对电场的抑制比微生物要强,热效应和溶液离子浓度对酶活的影响很小;不同介质环境的影响表明酶在环境中的三维结构状态是影响电场处理效果的最可能的原因。The effects of pulsed electric fields （PEF）on the activity oflipase were studied on this paper. Some affective factors such as electric field strength, number of pulses, treatment temperature and invironment of samples were changed. Results showed that： the treatment of PEF can cause the inactivity of lipase, and the inactivity rate increases with the increasing of the electric field strength and number of pulses. But the restrain of enzyme is stranger than microoganism. Temperature and ionic concentration have little effect on the activity of lipase. So some mechanisms can be deduced that structural change may be the major reason of lipase incativition by PEF.

关 键 词：高强脉冲电场 脂肪酶 酶活 

分 类 号：Q556[生物学—生物化学]