草鱼My5oD cDNA的克隆和序列分析  

Molecular Coning and Sequence Analysis of MyoD in Grass Carp (Ctenopharyngodon idella)

作　　者：王立新[1] 白俊杰[2] 叶星[2] 罗建仁[2] 陈宏[1] 简清[2] 劳海华[2] 

机构地区：[1]西北农林科技大学动物科技学院/陕西省农业分子生物学重点实验室,杨凌712100 [2]中国水产科学研究院珠江水产研究所/中国水产科学研究院热带亚热带鱼类选育与养殖重点开放实验室,广州510380

出　　处：《中国农业科学》2005年第10期2134-2138,共5页Scientia Agricultura Sinica

基　　金：中国水产科学研究院重点基金资助项目(99-08-02)

摘　　要：用RACE技术从受精后约22h的草鱼胚胎总RNA中扩增获得了草鱼MyoD基因的全序列,并对其序列进行了分析。结果表明,草鱼MyoD基因全长cDNA为1597bp,其中开放阅读框为825bp,共编码275个氨基酸,结构分析表明该肽链第1～84个氨基酸为草鱼MyoD基因的Basic区,第98～142个氨基酸为草鱼MyoD基因的HLH结构域,该序列所编码的肽链没有信号肽;通过对比分析已知的GeneBank中其它脊椎动物MyoD基因发现,该基因编码的氨基酸肽链随动物由低等向高等进化有加长的趋势,且核苷酸以及推测的氨基酸同源性和动物之间的亲缘关系相一致;草鱼MyoD基因的克隆为研究家鱼的肌肉发育调控的机理以及肉质改良奠定了基础。Total RNA was isolated from grass carp embryos at 22h post-fertilization to clone the MyoD cDNA by using rapid cDNA end amplification, and the sequence was analyzed with other animals. The results showed that： the full-length of grass carp MyoD is 1597 bp, with 825 bp open read frame enconding 275 amino acids, including a bHLH domain which composes of lth to 84th amino acids and 98th to 141th amino acids, without signal peptide; Compared grass carp MyoD with other animals, the results showed that the length of MyoD peptide is related to the evolution of animal, and the homology of nucleotide and amino acid accords with genetic relationship among the animals; Cloning of grass carp MyoD is meaningful for researching the mechanism of fish＇s muscle development and dominate, it is also helpful for improving the muscle quality of fish artificially.

关 键 词：草鱼 MyoD基因 克隆 全长CDNA 草鱼胚胎 序列分析 氨基酸同源性 RACE技术 脊椎动物 基因编码 

分 类 号：S965.112[农业科学—水产养殖]