里氏木霉91－3纤维素酶产生条件的研究  

Studies on the Conditions for Cellulase Production from Trichoderma reesei 91-3

作　　者：王成华[1] 孙纳新[1] 王健鹏 马梦瑞 韩东 

机构地区：[1]化工部饲料添加剂技术开发服务中心

出　　处：《食品与发酵工业》1996年第5期1-6,共6页Food and Fermentation Industries

基　　金：国家"八五"攻关项目

摘　　要：里氏木霉（Ｔｒｉｃｈｏｄｅｒｍａｒｅｅｓｅｉ）Ａ＿３经亚硝基胍和紫外线复合处理，获得一株纤维素酶高产菌株９１－３。该菌株在最适固态发酵条件下，纤维素酶滤纸酶活力为１７０ｕ／ｇ曲，产酶水平是出发菌株的１．６倍。酶作用的最适条件为ｐＨ４．８，５０℃；ｐＨ稳定范围为３～７；９０℃处理７ｍｉｎ，酶活保存率为９１．６４％；室温放置半年，酶活保存率在９０％以上，室温放置一年，酶活保存率在８０％以上。mutant strain 91-3 with high potency for cellulase production was obtained from Trichoderma reesei A3 which was treated with UV and NTG at room temperature.Cellulase Qu was obtained by growing the mutant strain on semi-solid state medium consisted of 3.5g of maize straw powder,1.5g of wheat bran,15ml of nutrition salt solution in 250ml of flasks at 30℃ for 96h. Enzyme activity of the Qu was 170u/g on filter paper.Cellulase activity was about 1.6 times that of the parent strain.The optimal conditions for enzyme action on filter paper were PH4.8 and 50℃.The enzyme was stable at PH 3.0～7.0.After incubating the enzyme at 90℃ for 7mins,90% of the enzyme activity remained.Put the enzyme at room temperature for one year, 80% of the enzyme activity remained.

关 键 词：里氏木霉 纤维素酶 91-3菌株 选育 发酵 酶制剂 

分 类 号：TQ925[轻工技术与工程—发酵工程]