反胶束体系的光密度与酶活性测定  

Optical density and enzyme activity in reverse micelles

作　　者：张东方[1] 初志战[1] 黄卓烈[1] 何勇虎[1] 朱磊[1] 

机构地区：[1]华南农业大学生命科学学院,广东广州510642

出　　处：《华南农业大学学报》2005年第4期88-91,共4页Journal of South China Agricultural University

摘　　要：以正庚烷-正丁醇-大豆卵磷脂配制反胶束,做了增溶不同量Tris-HCl缓冲液的试验. 结果表明:增溶不同水量的反胶束体系稳定性不一样,每3 mL增溶40～90 μL缓冲液的反胶束体系光密度在0～300 s内不断变化,透明澄清的稳定反胶束体系分布在每3 mL增溶0～40 μL及90～130 μL 2个区间. 增溶水量较少时,反胶束增溶含蛋白质与不含蛋白质水相的体系之间存在差别. 在水-正庚烷-正丁醇-大豆卵磷脂反胶束体系中未发现核糖核酸酶A1(Rnase A1)的超活性.Different amount of Tris-HCl buffer were solubilized in the reverse micelles made from heptanebutanol-lecithin. The optical densities of reverse micelles with 40 -90 μL aqueous phase per 3 mL were altering in 0 - 300 s. Clarified reverse micelles were stable with 0 - 40 μL and 90 - 130 μL aqueous phase per 3 mL. The reverse micelles with protein was different from that with buffer at low aqueous phase. There was no “superactivity” of ribonuclease A1 （ RNase A1 ） in the reverse micelles of waterheptane-butanol-lecithin.

关 键 词：反胶束 微乳 卵磷脂 核糖核酸酶A1 

分 类 号：Q55[生物学—生物化学] O647.11[理学—物理化学]