冷电弧技术杀灭水中微生物的实验研究  

Research on sterilization effect on liquid by cald plasma technology

作　　者：阮志农[1] 王中来[1] 陈昭华[1] 杨志超[1] 

机构地区：[1]福州大学生物科学与工程学院,福建福州350002

出　　处：《食品工业科技》2005年第11期76-79,共4页Science and Technology of Food Industry

基　　金：国家自然科学基金资助项目(20176006);福建省教育厅资助项目(JB03021)。

摘　　要：采用冷电弧技术杀灭连续流动的液体中的细菌和酵母菌。实验中采用自制的冷电弧杀菌装置(外观尺寸为80cm×17cm×1.3cm,放电面积为414cm2)杀灭菌液中的大肠杆菌987P(E.coli987P)、金黄色葡萄球菌(S.aureus)、枯草芽孢杆菌(B.subtilis)和白色念珠菌(C.albicans),结果表明,在液体流量为100mL/min的条件下,电压为20kV时杀灭E.coli987P和电压为25kV时杀灭S.aureus所需的时间均少于4s;电压为30kV时杀灭B.subtilis和C.albicans所需的时间也分别不超过12s和19s,相应的能耗仅分别为0.64、1.31、8.0和12.67kwh/m3。研究了电压、循环次数和空气流量等实验参数对杀菌效果的影响,结果表明,杀菌效果随着电压和循环次数的增加、空气流量的减少变得越来越好。通过对比处理前后E.coli987P及B.subtilis的透射电镜图,初步推测杀菌机理。该技术有望成为一种新型的食品工业冷杀菌技术。Cold plasma generated by dielectric barrier discharges was used to kill bacteria and yeast in flowing liquid in this study. E.coli 987P, S.oures, B. subtilis and C.albicars in suspension were treated with a home-made cold plasma sterilization device. It was shown that E.coli 987P was killed in less than 4s at the AC high voltage of 20kV and S.aureus was killed in less than 4s at the voltage of 25kV when the liquid flow rate was 100mL/min. Likewise, B. subtilis and C.albicars were killed in less than 12s and 19 s at the voltage of 30kV. Under the above conditions, the corresponding input power was only 0.64, 1.31, 8.0 and 12.67kwh/m^3. The influence upon sterilization effect by voltage, recycle times and atmosphere flow rate was studied. It was showed that the sterilization effect .became better with the increase of voltage and recycle time and the decrease of atmosphere flow rate. The sterilization mechanism was presumed through comparing Transmission Electron Microscope （TEM） photographs of the treated E.coli 987P and B.subtilis with the untreated ones. This technology has the potential to become a new type of non-thermal sterilization technology in food industry.

关 键 词：介质阻挡放电 水处理 冷电弧 杀菌 

分 类 号：TS205.9[轻工技术与工程—食品科学]