里氏木霉306生物合成组织型纤溶酶原激活剂(t-PA)5L罐分批发酵条件的研究  

Study on the Batch Fermentation Conditions of Tissue-type Plasminogen Activator (t-PA) Producing by Trichoderma reesei 306

作　　者：江洁[1] 杜连祥[1] 路福平[1] 邹亚杰[2] 肖琳[2] 乔玉龙[2] 张宝涛[2] 

机构地区：[1]天津科技大学生物工程学院,天津300222 [2]齐齐哈尔大学生命科学与工程学院,齐齐哈尔161006

出　　处：《食品与发酵工业》2005年第11期18-21,共4页Food and Fermentation Industries

基　　金：黑龙江省自然科学基金(No.D0352)

摘　　要：以摇瓶分批发酵条件为基础，对基因工程菌株里氏木霉(Trichodermareesei)306进行了5L发酵罐分批发酵条件的研究。确定了适宜的发酵条件：温度为28℃，溶氧浓度控制30％饱和度，pH值在自然状态下进行发酵。以5L发酵罐分批发酵试验数据为依据，对里氏木霉306生物合成t-PA分批发酵动力学进行了研究。应用MATLAB工具软件进行非线性规划，建立了菌体生长、底物消耗和产物合成的发酵动力学模型。In the paper, we studied the fermentation conditions and the batch fermentation kinetics of tissuetype plasminogen activator producing by recombinant Trichoderma reesei 306 . Based on the optimal conditions obtained in shake-flask, the batch fermentation was performed in 5 L fermentor. The optimized fermentation conditions are：temperature 28℃ ,dissolved oxygen 30 % saturated concentration, pH natural. The batch fermentation kinetics of t-PA producing by Trichoderma reesei 306 were studied based on the experimental data from the fermentation in 5 L fermentor. The kinetic models of fermentation were established by using MATLAB software.

关 键 词：里氏木霉 组织型纤溶酶原激活剂(t—PA) 分批发酵 发酵动力学模型 

分 类 号：TS201.3[轻工技术与工程—食品科学]