产β-葡聚糖酶高产菌株的驯化及筛选  

The Acclimatization and Selection of Strain Containing Abundant β-Glucanase

作　　者：李春磊[1] 任海霞[1] 王瑞明[1] 关凤梅[1] 

机构地区：[1]山东轻工业学院食品与生物工程学院,山东济南250100

出　　处：《现代食品科技》2006年第1期23-25,共3页Modern Food Science and Technology

摘　　要：根据刚果红与β-1,3、β-1,4-葡聚糖紧密结合而保持红色的特征,筛选β-葡聚糖酶高产菌株。本实验室保存的黑曲霉QYW-01经紫外诱变和产酶驯化,获得一株高产β-葡聚糖酶的黑曲霉QYW-01A06,在摇瓶条件下发酵液酶活力达2642U/mL,是出发菌株的2.4倍。The strain containing abundant 13-glucanase was selected according to the reaction of β-glucan with Congo-red can produce red pigment In this paper, we obtained a strain containing abundant β-glucanase （Aspergillus niger QYW-01A06） bred by UV and acclimatized from its parent strain QYW-01. the result showed that the activity of β-glucanase of QYW-01A06 could reach 2642u/mL, it was 2.4 times of QYW-01.

关 键 词：黑曲霉 Β-葡聚糖酶 刚果红 驯化 筛选 

分 类 号：TQ925[轻工技术与工程—发酵工程]