胃癌中PTEN突变在其蛋白异常表达中的作用  

Role of mutation in abnormal protein expression of PTEN in gastric carcinoma

作　　者：刘淑芳[1] 朱忠政[2] 王爱忠[2] 贾杭若[2] 金夏祥[2] 钱珍华[1] 

机构地区：[1]同济大学附属铁路中心医院消化内科 [2]解放军第113医院病理科,浙江宁波315040

出　　处：《中国癌症杂志》2006年第1期28-30,共3页China Oncology

摘　　要：背景与目的:胃癌组织中PTEN蛋白表达缺失率较高,本文旨在探讨胃癌组织中PTEN基因突变在其蛋白异常表达中的作用。方法:应用PCR-SSCP-DNA测序和免疫组织化学技术检测53例胃癌组织中PTEN基因突变及其蛋白表达水平的改变。结果:53例胃癌组织中共检测到5例PTEN基因突变,突变率仅为9.4%,低于PTEN蛋白表达缺失率(34.0%)。此5例突变阳性胃癌组织中的PTEN蛋白表达情况为4例阴性、1例弱阳性。结论:基因突变是胃癌组织中PTEN蛋白异常表达的原因之一,突变以外的其他异常改变可能在这一过程中发挥了更为重要的作用。Background and Purpose： Abnormal expression of PTEN protein occurs commonly in gastric carcinoma. Our aim was to study the role of PTFEN gcne mutation in abnormal protein expression in gastric carcinoma. Methods： Fiftythree gastric carcinoma samples with normal tissue beside tumors were collected. The mutations of cxon 5 - 8 of PTEN gene were examined by polymerasc chain reaction-single strand conformation polymorphism （PCR-SSCP） and DNA sequencing analysis. The expression of PTEN protein was evaluated by immunohistochcmistry method. Results： The mutation rate of PTEN gene was 9.4% （5/53）, which was lower than the protein expression deletion rate （34.0%）. Among those 5 mutation-positive samples, 4 were negative and 1 was weakly positive for PTEN protein expression. Conclusions： Mutations may contribute to abnormal protein cxpressinn of PTEN in gastric carcinoma, but some changes other than mutation might play more important roles in this process.

关 键 词：胃肿瘤 PTEN基因 突变 蛋白表达 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]