Expression of Tyrosine-kinase Receptors and Neurotrophins in Human Neuroblastomas  

作　　者：Daojian Rong Haicle Gao Jinhua Zhang Xinyuan Li Xu Li Jihong Zhang 

机构地区：[1]Minimally Invasive Surgery Center, theAffiliated Zhong Shan Hospital, Dalian University, Dalian 116001, China. [2]Laboratory of Paediatric Surgery, theSecond Affiliated Hospital, ChinaMedial University, Shenyang 110004,China.

出　　处：《Chinese Journal of Clinical Oncology》2005年第6期852-855,共4页中国肿瘤临床（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China （No.39470739）.

摘　　要：OBJECTIVE The aim of this study was to investigate mRNA expression of tyrosine-kinase receptors （TRKs） and neurotrophins （NTs） in human neuroblastomas. METHODS Expression of TrkA, TrkB, TrkC and BDNF was quantitatively examined by reverse transcription-polymerase chain reaction （RT-PCR） in 27 cases of neuroblastomas. RESULTS The high and total rates of TrkA were expressed in significantly more tumors in a lower-stage group compared to a higher-stage group （P〈0.05） and the high level of TrkA expression was correlated positively with the 2-year cumulative-survival rate of the patients （P〈 0.01）. The high and total rates of TrkB were expressed in significantly more tumors in a higher-stage group compared to a lower-stage group （P〈0.05）. All 3 rates of BDNF expression between the 2 groups showed no statistical difference （P〉0.05）, but the co-expression ratio of TrkB and BDNF showed a remarkable significance in the higher-stage group more than in the lower-stage group （P〈0.05）. TrkC expression was usually accompanied by TrkA expression, but there was only a non-significant trend between TrkC expression and TrkA expression. CONCLUSION RT-PCR for mRNA expression of TRKs and NTs has important clinical significance relating to the tumor stage and outcome for patients with neuroblastomas.

关 键 词：NEUROBLASTOMA tyrosine-kinase receptor NEUROTROPHIN RTPCR. 

分 类 号：R730.264[医药卫生—肿瘤]