Co^(60)诱变原生质体选育高产酒精酵母  

Breeding of High Yield Alcohol Yeast from Protoplast by Co^(60) Mutagenesis

作　　者：张伟[1] 朱会霞[2] 李英军[1] 刘卫华[1] 丁鑫[1] 孙金旭[2] 李长文[1] 

机构地区：[1]河北农业大学食品科技学院,河北保定053000 [2]河北省衡水学院,河北保定053000

出　　处：《酿酒科技》2006年第1期23-26,共4页Liquor-Making Science & Technology

基　　金：河北省科技攻关项目(032201100)资助

摘　　要：将较高产酒精酵母制备成原生质体,经Co60诱变后,采用四级筛选,得到高产酒精酵母菌株Co-158,其遗传性状稳定。以玉米粉糖化醪为基质发酵72h,成熟醪酒精体积分数为17.8%(v/v),残还原糖为0.0137%。Co-158菌株成熟醪酒精体积分数比出发菌株提高了16.34%,比ADY提高了24.48%,残还原糖含量亦远远低于出发菌株和ADY。High yield alcohol yeast strain Co-158 of steady genetic determinant was obtained from protoplast by Co^60 mutagenesis and guartemary screening, after inducing the protoplast prepared with high alcohol yield yeast by Co^60. After 72 h fermentation of com flour saccharifying mash as substrate, alcohol yield reached 17.8 % （v/v） and residual reducing sugar content was 0.0137 %. Alcohol yield by Co-158 strain was 16.34 % higher than that by starting strain and 24.48 % higher than that by ADY. Besides, residual reducing sugar content by Co-158 sWain was far lower than that by starting strain or ADY.

关 键 词：酒精酵母 Co^60诱变 原生质体 选育 

分 类 号：TS261.11[轻工技术与工程—发酵工程]