d－生物素代替玉米浆发酵生产谷氨酸的研究  

Study on SUbstitution of d-Biotin for Corn Steep Liquor in Fermentative Production of Glutamic Acid

作　　者：梁新梅 孙吉臻 张跃庆 王丽谦 董金兰[1] 徐娉婷[1] 

机构地区：[1]北方味精厂,哈尔滨师范大学生物系,黑龙江省科学院应用微生物所

出　　处：《生物技术》1996年第2期30-34,共5页Biotechnology

摘　　要：用ｄ－生物素代替玉米浆的摇瓶发酵实验，筛选出高产酸菌株，测定了ｄ－生物素的亚运量可变范围。比较了单一玉米浆、单一ｄ－生物素与两者各半量发酵结果及不同接种量对产酸的影响。采用ｄ－生物素代替半量玉米浆的中糖发酵工艺应用于生产。结果证明此工艺可行，降低了成本，提高了产酸率，平均产酸率达７．８１％，平均转化率达５３．２％。With the fermentative experiments of shake flask in which corn steep liquor was replaced by d-biotin,the high acid-produced strains were selected and the variable range of sub opportune amount of d-biotin was determined.By comparing the fermentative results in using single corn steep liquor,Singte d-biotin and both in half amount.and the effects of different inoculating amount on producing acid,the medium-sugar fermentative technology of substitution of d-biotin for half-amount corn steep liquor was used in production.This technology has been proved feasible by its decreasing cost and increasing acid-produced rate.Average acid-produced rate is 7.81 % and average transformation is 53.2 %.

关 键 词：谷氨酸 发酵 D-生物素 玉米浆 

分 类 号：TQ922.1[轻工技术与工程—发酵工程]