膳食多不饱和脂肪酸构成对小鼠淋巴细胞功能及脂肪酸构成的影响  

Effect of dietary polyunsaturated fatty acid composition on function of spleen lymphocyte and fatty acid composition in mice

作　　者：潘妹霞[1] 苏宜香[2] 

机构地区：[1]广东省人民医院营养科,广州510080 [2]中山大学公共卫生学院

出　　处：《广东医学》2006年第2期172-174,共3页Guangdong Medical Journal

基　　金：广州市科技局研究项目(编号:2002L2C0081)

摘　　要：目的观察膳食n-6/n-3PUFA比值对淋巴细胞脂肪酸构成和细胞功能的影响。方法48只BALB/c小鼠随机分为4组,即n-6/n-3比值分别为1,7.5,15,30;基础饲料采用AIN-93G配方,S:M:P为1:1.5:3.7。饲养12周。测定小鼠T淋巴细胞功能、IL-2水平和淋巴细胞脂肪酸构成。结果n-6/n-3比值接近1时,小鼠T淋巴细胞增殖活性及培养上清IL-2水平显著降低;淋巴细胞C18:3,C20:6,n-3PUFA中含量显著减少;淋巴细胞C22:6含量与淋巴细胞增殖活性显著负相关,C18:3,C20:5,C22:6含量与CD4+T淋巴细胞比例显著负相关;淋巴细胞脂肪酸构成的改变基本与膳食脂肪酸的改变基本一致。结论膳食S:M:P为1:1.5:3.7时,n-6/n-3比值为1时,小鼠T淋巴细胞增殖活性受到抑制,其作用可能与IL-2的降低有关;小鼠脾淋巴细胞的脂肪酸构成受膳食脂肪酸构成的影响。Objective To study the effect of dietary n-6/n-3 PUFA ratio on function of spleen lymphocyte and fatty acid composition in mice.Methods 48 male BALh/c mice were divided into 4 groups, according to the n-6/n-3 PUFA ratio of 1, 7.5, 15 and 30, respectively. The dietary S：M：P was 1 ： 1.5：3.7 shmilar to AIN-93G formula. After 12 weeks of breeding, all mice were sacrificed. The function and fatty acid composition of lymphocyte, IL-2 concentration were raeasured. Resuits When dietary n-6/n-3 ratio approximated 1 , the lymphocyte proliferatian and IL-2 level significantly decreased; The concentration of C18：3,C20：6 and n-3 PUFA in lymphocyte significantly decreased; The concentration of C22：6 in lymphocytes was negatively correlated with the proliferation of spleen lymphocytes. The concentration of C18：3, C20：5 and C22：6 in lymphocyte was negatively correhted with the proportiom of CD4^＋ T cell. Conclusion In diet with 1 ： 1.5：3.7 of S： M： P, T lymphocyte function of mice is suppressed when dietary n-6/n-3 ratio approximates 1, which might be related to decrease in IL-2. The fatty acid composition of lymphocyte is affected by diets in mice.

关 键 词：脂肪酸构成 多不饱和脂肪酸 免疫 

分 类 号：R57[医药卫生—消化系统] R511.6[医药卫生—内科学]