啤酒对血清酶活性影响的体内外实验(英文)  

In vivo and in vitro experiment on the effect of beer on the serum enzyme activity

作　　者：顾洪雁[1] 陶如[1] 翟静[1] 孙凌云[1] 王涛[1] 柏素云[1] 

机构地区：[1]泰山医学院生物化学教研室,山东省泰安市271000

出　　处：《中国临床康复》2006年第4期190-192,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:观察体内外实验中受试者饮用啤酒后各种血清酶活性的变化情况。设计:观察对比实验。单位:泰山医学院基础医学研究所。对象:实验于2005-03/04在泰山医学院基础医学研究所完成。选择泰山医学院学生17名,年龄19～35岁,包括本科生和研究生,实验前均签署同意书。方法:①体内实验:受试者统一正常饮食后3h采静脉血3mL,作为对照。然后立即口服啤酒4mL/kg,分别于15,30,45,60,90,120,180min后各采血3mL,测定血液中丙氨酸氨基转移酶、天冬氨酸氨基转移酶、γ-谷氨酰转肽酶、碱性磷酸酶、肌酸激酶、乳酸脱氢酶、淀粉酶、脂肪酶活性的变化。②体外实验:选取17份新鲜受试者血清,分别加入两个试管中,每管0.5mL。对照管加入20μL生理盐水;测定管中加入20μL啤酒溶液,观察啤酒对以上各种酶活性的直接影响。主要观察指标:体内外实验中血清丙氨酸氨基转移酶、天冬氨酸氨基转移酶、γ-谷氨酰转肽酶、碱性磷酸酶、肌酸激酶、乳酸脱氢酶、淀粉酶及脂肪酶的活性。结果:纳入17名学生,全部进入结果分析,无脱落。①体内实验:啤酒可显著降低血清天冬氨酸氨基转移酶活性(418.08±58.68,383.41±63.01)nkat/L,显著升高血清碱性磷酸酶活性(3678.57±436.25,3962.96±400.91)nkat/L(χ2=19.00～20.00,P<0.01),其余酶活性均有不同程度的升高。②体外实验:啤酒在体外对各种酶活性均有一定程度的抑制作用。结论:啤酒在体内外对酶活性均有一定影响,从而影响机体代谢,过量饮用会影响健康。在常规血清酶学检测中,应避免患者饮用啤酒所造成的干扰,以确保实验结果的准确可靠。BACKGROUND： There have been no reports available about the direct effect of beer on serum enzyme activity. OBJECTIVE： To observe the changes of the activity of various serum enzymes of the subjects in the in vivo and in vitro experiments after drinking bear. DESIGN： An observational controlled experiment. SETTING： The Institute of Basic Medicine of Taishan Medical College. PARTICIPANTS： The experiment was performed at the Institute of Basic Medicine of Taishan Medical College between March 2005 and April 2005. We selected 17 college students, aged 19 to 35 years, from Taishan Medical College, including undergraduate students and graduate students. Informed consents were obtained from the subjects before the experiment was conducted. METHODS： ① In vivo experiment： 3 mL of venous blood was collected from the subjects 3 hours after the ordinary diet as the control. Then, the subjects drank beer at an amount of 4 mL/kg according to their body mass immediately. 3 mL of blood was collected respectively 15, 30, 45, 60, 90, 120, 80 minutes later to measure the changes of the activity of alanine aminotransferase, aspartate aminotransferase, γ-glutamyl transpeptidase, alkaline phosphatase, ereatine kinase , lactate dehydrogenase, diastase and llpase. ② In vitro experiment： 17 fresh serum samples were added into two test tubes separately with 0.5 mL of serum in each tube. 20μL of normal saline was added to the tube of the control and 20 μL of beer was added into the test tube. The direct effect of beer on the activity of various enzymes was observed. MAIN OUTCOME MEASURERS： The changes of the activity of alanine aminotransferase, aspartate aminotransferase, γ-glutamyl transpeptidase, alkaline phosphatase, ereatine kinase , lactate dehydrogenase, diastase and lipase of the serumon the in vivo and in vitro experiment. RESULTS： Totally 17 students were involved and all the students entered the stage of result analysis with no loss in the midway. ① In vivo experiment： Beer significa

关 键 词：啤酒 酶学 血液 血清 酶活性 体内 实验 体外 

分 类 号：R-33[医药卫生]