杂色云芝漆酶用于提取人参总皂甙的研究  

Extraction Study on Ginsenosides with Laccase

作　　者：郭梅[1] 白东清[2] 蒲军[2] 杜连祥[2] 路福平[2] 

机构地区：[1]天津农学院食品科学系,天津300384 [2]天津科技大学生物工程学院工业微生物重点实验室

出　　处：《食品科学》2006年第2期166-168,共3页Food Science

摘　　要：采用正交试验,对水浸提取条件、漆酶酶解条件进行了研究。结果表明人参总皂甙最佳提取工艺为:先用漆酶酶解,每克人参加入15ml漆酶粗酶液(92U/ml),在30℃、pH3.5的条件下酶解1.5h,然后水浸提取人参总皂甙。经过漆酶酶解处理后人参总皂甙提取率比水浸提取法提高了70.9%。The optimum conditions of enzymolysis and extraction process by orthogonal design were studied. The results showed that the optimum conditions of ginsenosides extraction technique were as follows： First, laccase treatment： 1g Panax ginseng was put into 15ml （92U/ml） laccase with temperature 30℃, pH value 3.5 and fermenting time 1.5h. Then, in comparison with water-extraction, the enzyme-based extraction rate of ginsenosides is increased by 70.9%.

关 键 词：漆酶 酶解 人参总皂甙 正交试验 

分 类 号：R282.71[医药卫生—中药学]