比色法快速测定糙米中γ-氨基丁酸含量研究  

Spectrophotometry Determination of γ-Aminobutyric Acid in Brown Rice

作　　者：陈恩成[1] 张名位[2] 彭超英[1] 张瑞芬[2] 张雁[2] 池建伟[2] 

机构地区：[1]广东省农业科学院生物技术研究所农业部功能食品重点开放实验室,广州510640 [2]华南理工大学轻工食品学院,广州510641

出　　处：《中国粮油学报》2006年第1期125-128,共4页Journal of the Chinese Cereals and Oils Association

基　　金：广东省国际合作项目(NO:2003C50210);广州市应用基础研究项目(NO:2005J1-C0181)

摘　　要：基于Berthelot显色反应,研究了快速测定糙米中γ-氨基丁酸含量的比色法。结果表明,糙米或发芽糙米粉以60%乙醇溶液为溶剂,在70℃温度下回流提取2 h,其离心上清液以重蒸苯酚和次氯酸钠溶液作为显色剂显色反应,在645 nm处比色测定,以标准品为对照可以计算样品中γ-氨基丁酸含量。该方法简单、快速、经济,相对标准偏差(RSD)为0.98%,平均回收率为99.4%,适合于大量稻米品种中γ-氨基丁酸的定量分析。The spectrophometric determination of γ-Aminobutyric acid in brown rice was studied based on Berthelot reaction. A good linear relationship was obtained between the OD value and the concentration of GABA. The linear equation is γ=0.9116x-0.013 ;the RSD is 0.98% and the average recovery is 99.4%. This method is simple, rapid and economical, and thus is recommended as a conventional method of detecting GABA in brown rice for a large quantity rice variety.

关 键 词：分光光度法 Γ-氨基丁酸 糙米 测定 比色法 

分 类 号：TS21[轻工技术与工程—粮食、油脂及植物蛋白工程] TS201.24[轻工技术与工程—食品科学与工程]