基于16s rDNA的PCR快速鉴定啤酒腐败菌的研究  

Rapid detection of PCR on beer spoilage-bacteria based on 16s rDNA

作　　者：田小群[1] 周世宁[1] 

机构地区：[1]中山大学生命科学学院

出　　处：《酿酒》2006年第2期51-54,共4页Liquor Making

摘　　要：从Genebank中下载啤酒腐败菌所在属的63种细菌的16s rDNA序列,用DNAstar软件进行序列对比分析,找出这63种细菌16s rDNA序列所共有的5条同源序列,以这5条同源序列为基础设计出一对适合所有腐败菌的通用引物:上游:5’-GAACAGGATTAGATACCC-3’,下游:5’-GACTTAACCCAACATCTCAC-3’。利用该对引物,可以检测出啤酒厂出现的8种腐败菌,而对啤酒厂出现的13种非腐败菌则不能检出,保证了该引物对啤酒腐败菌的特异性。According to literatures and all kinds of beer - spoilage, 16s rDNA sequences of 63 species of beer - spoilage strains were downloaded from Genebank. By analyzing and contrasting these sequences with DNAstar soft-ware, 5 common sequences were found among the 63 sequences of 16s rDNA. On the base of these 5 common sequences, a pair of primers was designed which fit all beer- spoilages. The primer pair includes a forward one（5＇ - GAAC AGGATTAGATACCC - 3＇）and a reverse one （5＇ - GACTTAAC CCAACATCTCAC - 3＇）. This primer pair was specific to beer-spoilages but not specific to non - spoilages found in brewery.

关 键 词：啤酒腐败菌 快速鉴定 引物设计 

分 类 号：TS262.3[轻工技术与工程—发酵工程] F810.42[轻工技术与工程—食品科学与工程]