N^+注入诱变选育氨肽酶高产菌株及发酵条件初步优化  

Breeding of Aminopeptidase-producing Strains by N^+ Implantation and Preliminary Research on Fermentation Conditions

作　　者：吴庆勋[1] 谷海先[1] 赵光鳌[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室

出　　处：《食品与发酵工业》2006年第1期15-18,共4页Food and Fermentation Industries

摘　　要：通过N+注入米曲霉WJ05-1,筛选得到2株氨肽酶活力提高了30%的高产菌株M60-5-13和M80-10-7,经多次传代实验表明2菌株遗传稳定性良好。对M80-10-7的发酵条件,如碳氮源及浓度、起始pH、发酵时间和表面活性剂等进行了初步优化,进一步使产酶水平提高了77.5%。Two high-yield strains, M60-5-13 and M80-10-7, were obtained producing aminopeptidase through N^＋ implanting into Aspergillus oryzae WJ05-1. The yields increased by 30% than that of the original. It is seen that these two strains have good genetic stability after five times of subculture. The culturing conditions including initial pH, fermentation time and surfactant effect on fermentation of M80-10-7 were studied. The optimal carbon and nitrogen source and their optimal concentrations were also determined in this study. Under the optimum condition, the yield of aminopeptidase increased by 77.5 %.

关 键 词：N^+注入 诱变育种 氨肽酶 米曲霉 发酵优化 

分 类 号：TS201.25[轻工技术与工程—食品科学]