cDNA Cloning, Prokaryotic and Eukaryotic Expression and Characterization of Porcine Leukemia Inhibitory Factor  

作　　者：LI Ming-tang JIANG Yong WANG Shu-mei LI Yong-ming WANG Fang HOU Xia YANG Hong MA Tong-hui 

机构地区：[1]Membrane Channel Research Laboratory, Northeast Normal University, Changchun 130024, P. R. China [2]Department of Anesthesia, Second Teaching Hospital, Jilin University, Changchun 130041, P. R. China

出　　处：《Chemical Research in Chinese Universities》2006年第2期145-149,共5页高等学校化学研究（英文版）

基　　金：SupportedbytheNationalNaturalScienceFundforDistinguishedYoungScholars(No.30325011),theNationalNaturalScienceFoundationofChina(Nos.30470405and30570864),DistinguishedYoungScholarsFundofJilinProvince(No.20030112)andExcellentYoungTeachersProgramofMOE,P.R.China.

摘　　要：Molecular cloning of the porcine leukemia inhibitor factor（pLIF） has not been reported. A full-length eDNA encoding pLIF was cloned, expressed and characterized. The full-length porcine LIF cDNA encodes a 202 amino acid protein that has an 84% sequence identity to mouse LIF and 86% sequence identity to human LIF. The deduced amino acid sequence of a pLIF protein contains six conserved consensus N-linked glycosylation sites and six cysteine groups to form potential disulfide bonds. The pLIF was expressed in E coli, as a mature form, and in CHO cells as a secreted form. Both the forms of the recombinant pLIFs can maintain murine embryonic stem cells in an undifferentiated state in a culture. The recombinant pLiFs will be useful in establishing a long-term culture of stable pluripotent porcine embryonic stem cells for further manipulation.Molecular cloning of the porcine leukemia inhibitor factor（pLIF） has not been reported. A full-length eDNA encoding pLIF was cloned, expressed and characterized. The full-length porcine LIF cDNA encodes a 202 amino acid protein that has an 84% sequence identity to mouse LIF and 86% sequence identity to human LIF. The deduced amino acid sequence of a pLIF protein contains six conserved consensus N-linked glycosylation sites and six cysteine groups to form potential disulfide bonds. The pLIF was expressed in E coli, as a mature form, and in CHO cells as a secreted form. Both the forms of the recombinant pLIFs can maintain murine embryonic stem cells in an undifferentiated state in a culture. The recombinant pLiFs will be useful in establishing a long-term culture of stable pluripotent porcine embryonic stem cells for further manipulation.

关 键 词：Leukemia inhibitor factor PORCINE CLONING Recombinant protein Embryonic stem cell PLURIPOTENCY 

分 类 号：R733.7[医药卫生—肿瘤]