大豆异黄酮糖苷酶的发酵和纯化  

Fermentation and Purification of Soybean Isofiavone Glucoside Hydrolase

作　　者：马达[1] 刘冰[1] 芦明春[1] 鱼红闪[1] 金凤燮[1] 

机构地区：[1]大连轻工业学院生物与食品工程学院

出　　处：《食品与发酵工业》2006年第2期61-63,共3页Food and Fermentation Industries

基　　金：国家自然科学基金(No.30371744;20476017;30470055)

摘　　要：大豆异黄酮糖苷酶可水解大豆异黄酮的糖基使其生成苷元,从而提高大豆异黄酮的生物活性。实验中筛选得到了大豆异黄酮糖苷酶产酶菌株 Absidia sp．R,确定了该菌株发酵产酶培养基。采用硫酸铵沉淀和离子交换法对该酶进行了纯化,并初步确定了其分子质量。Genistin and daidzin were hydrolyzed by the soybean isofiavone glucoside hydrolase to form genistein and daidzein so asto increase their biological activity. Absidia sp. R, which produces soybean isofiavone glucoside hydrolase, was obtained and its fermentation conditions was optimized. The enzyme was purified by （NH4）2SO4 Precipitation and DEAE - Cellulose ion exchange chromatographic colum. Its molecular weight was also assayed in this paper.

关 键 词：大豆异黄酮糖苷酶 发酵 纯化 

分 类 号：TQ464.8[化学工程—制药化工]