低能离子注入在CGTase高产菌株选育中的应用  

Application of low energy ion implantation in breeding of high yield CGTase strains

作　　者：王雁萍[1] 段宇珩[1] 许平辉 秦广雍[1] 霍裕平[1] 

机构地区：[1]郑州大学河南省离子束生物工程重点实验室,郑州450052 [2]郑州市医药科技开发中心,郑州450001

出　　处：《工业微生物》2006年第1期23-25,共3页Industrial Microbiology

基　　金：河南省科技攻关项目(001160201)

摘　　要：应用能量30keV、剂量为5×1015N+/cm2的离子注入对环糊精葡萄糖基转移酶产生菌进行诱变育种,得到液体发酵24h产酶高于出发株50%以上的菌株11株。5代传代试验表明诱变后得到的高产菌株产酶具有较好的传代稳定性。Low energy ion beam （5×10^15 N^＋/cm^2, 30keV） was applied as mutagenic agent in the breeding of high yield cyclodextrin glucanotransferase strains. 11 strains were obtained with yield of 50 % higher than that of parent strain after cultivated for 24h. The stability of high yield strains were observed. The results showed that the strains were stable in the production of cyclodextrin glucanotransferase after 5 generations.

关 键 词：离子注入 诱变育种 环糊精葡萄糖基转移酶 

分 类 号：TQ925[轻工技术与工程—发酵工程]