华北地区汉族群体15个短串联重复序列基因座遗传多态性分析  

Fifteen short tandem repeat polymorphisms in Han race of North China

作　　者：范新萍[1] 丁洁[1] 梁燕[1] 肖白[1] 周艳[1] 刘敬忠[1] 

机构地区：[1]首都医科大学附属北京朝阳医院基础医学研究中心,北京100020

出　　处：《中华医学遗传学杂志》2006年第2期227-229,共3页Chinese Journal of Medical Genetics

摘　　要：目的调查华北地区汉族人群15个短串联重复序列（short tandem repeat，STR）基因座遗传多态性分布和群体遗传学数据。方法应用毛细管电泳技术和五色荧光复合扩增的方法，检测597名汉族无关个体的15个STR基因座基因型。结果15个STR基因座的基因频率分布均符合Hardy-Weinberg平衡。所检测的15个STR遗传标记均具有高度多态性，杂合度均超过0．62，15个基因座的个体识别力在0．802-0．967之间，非父排除率在0．320～0．697之间，匹配概率在0．033～0．198之间。15个基因座的累积个体识别能力为0．999999以上，累积非父排除率为0．99999571，累积匹配概率为8．93×10^-18。结论联合检测15个基因座可为亲缘鉴定和个体识别提供可靠的法医学证据，这15个STR基因座适用于中国人群的法医物证学检验。Objective To research on the genetic polymorphism distributions of 15 short tandem repeat（STR） loci in Han race of North China and the genetic data of population genetics. Methods The capillary electrophoresis and five-color fluorescent multi-amplifying were applied to detect the genotypes of 15 STR loci in 597 unrelated Chinese Han individuals. Results No significant deviation from the Hardy-Weinberg Equilibrium was observed. High polymorphism was detected in the loci. Statistical analysis was carried out to obtain some parameters of forensic medicine. The beterozygosity of 15 loci was above 0.62. The values of discrimination power （DP） at 15 STRs ranged from 0.802 to 0.967. The values of excluding probability of paternity （EP） ranged from 0. 320 to 0. 697. The values of probability matching （Pm） ranged from 0.033 to 0.198. The fdteen loci showed an accumulated total discrimination power （TDP） more than 0.999999, a cumulative excluding probability of patemity（CEP） as 0.99999571, and total probability matching to be 8.93 × 10^-18. Conclusion The data indicated that detecting combined 15 STRs is sensitive and reliable, and can be used to forensic and individual identification cases in Chinese group.

关 键 词：短串联重复序列 遗传多态性 等位基因频率 

分 类 号：R394[医药卫生—医学遗传学] R394.6[医药卫生—基础医学]