碳源对苏云金杆菌HD224菌产几丁质酶的影响  

The effect of carbohydrate resources on the synthesis of chitinase by Bacillus thuringiensis HD224 strain

作　　者：林毅[1] 洪雪梅[2] 彭锟[1] 

机构地区：[1]华侨大学生物工程与技术系,福建泉州362021 [2]华侨大学信息科学与工程学院,福建泉州362021

出　　处：《食品工业科技》2006年第3期98-99,共2页Science and Technology of Food Industry

基　　金：福建省自然科学基金(B0510011);福建省青年科技人才创新基金(2003J024);华侨大学科研启动基金

摘　　要：考察八种碳源对苏云金杆菌HD224菌产几丁质酶的影响。几丁质为其最适碳源,2.0%粉末几丁质最有利于产酶,而同浓度的胶体几丁质其诱导效果远不如粉末几丁质。N-乙酰葡萄糖胺和葡萄糖的存在使HD224菌的产几丁质酶系统完全受到了抑制。麦芽糖、乳糖、淀粉和蔗糖使其产酶量发生不同程度的降低;而麸皮的加入使菌株的产酶能力显著提高。The synthesis of chitinase by Bacillus thuringiensis HD224 strain using eight different carbohydrate resources was studied. As the optimal carbohydrate, chitin powder at 2.0% had the best production of chitinase, while colloidal chitin at 2.0% was inferior. No chitinase activity was detected at the presence of NAG or glucose. The other five carbohydrate resources such as starch inhibited the production of chitinase.

关 键 词：苏云金杆菌 几丁质酶 碳源 

分 类 号：TS201.25[轻工技术与工程—食品科学]