FMOC柱前衍生-高效液相色谱法测定饮料中的牛磺酸  

DETERMINATION OF TAURINE IN BEVERAGES BY HIGH PERFORMANCE LIQUID CHROM ATOGRAPHY AFTER DERIVATIZATION WITH 9-FLUOLENYLMEGHYL CHLOROFORMATE.

作　　者：王晓莺[1] 孙成均[1] 杨元[2] 张立实[1] 

机构地区：[1]四川大学华西公共卫生学院,成都610041 [2]成都市疾病预防控制中心

出　　处：《现代预防医学》2006年第3期371-373,共3页Modern Preventive Medicine

摘　　要：目的：建立饮料中牛磺酸的高效液相色谱测定方法.方法：以9-氯甲酸芴甲酯（FMOC）为柱前衍生剂,与饮料中的牛磺酸发生衍生化反应后,反相高效液相色谱法测定.以甲醇-0.025 mol/L磷酸盐缓冲溶液为流劝相（pH=8.0）,C18柱为分离柱,二极管阵列检测器（265 nm）检测.结果：本法相对标准偏差为3.38%,加标回收率范围为95.64%～108.3%,检出限为46ng.结论：本方法快速、简单、准确度高,适合于保健食品类饮料中牛磺酸的测定.Objective To establish a high performance liquid chromatographic （HPLC） method for the determination of taurine in beverages. Methods： After derivatization with FMOC, tarine in beverages was separated and quantitied by HPL,C. A mobile phase of methanol - 0.025 mol/L phosphate buffer （pH= 8.0）, an analytical column of C48 and UV detection wavelength of 265 nm were used in the study. Results： The recoveries ranged from 95.64%--108.3%, RSD was 3.38% and detection limit was 46 ng for this method. Conclusion： The method is reliable, accurate and suitable to the determination of taurine in beverages.

关 键 词：牛磺酸 高效液相色谱法 柱前衍生 

分 类 号：R155.5[医药卫生—营养与食品卫生学]