高效液相色谱法快速测定海产品中牛磺酸  

Fast determination of taurine in marine products by high performance liquid chromatography

作　　者：张晾[1] 耿越[1] 张静静[1] 涂文利[1] 

机构地区：[1]山东师范大学生命科学学院动物抗性重点实验室,山东济南250014

出　　处：《食品与药品》2006年第04A期56-58,共3页Food and Drug

基　　金：山东省优秀中青年科学家奖励基金资助项目(No.109034)

摘　　要：目的建立用高效液相色谱快速测定海产品中牛磺酸的方法。方法将长竹蛏去壳,经匀浆、水煮、离心后用磺基水杨酸沉淀蛋白,采用邻苯二甲醛柱前衍生,通过EclipseZorbaxC18柱进行分离、330nm波长测定牛磺酸含量。结果此法平均回收率为101.3%,精密度、灵敏度较高,其它成分不干扰本测定。结论此法可以快速测定海产品中的牛磺酸。Objective To establish the method for fast determination of taurine in marine products by high performance liquid chromatography（HPLC）, Methods Solens were shelled, then homogenized, boiled and centrifugated. Proteins in solution were deposited by 5-sulfosalicylic acid. The content of taurine in solen was determined by high performance liquid chromatography at the wavelength of 330 nm through Eclipse Zorbax C18 column with pre-column opthaldialdehyde derivatization. Results The proposed method had high precision and sensitivity and was not interfered by other complex components. The average recovery was 101.3%. Conclusion This method can be used for fast determination of taurine in marine products.

关 键 词：高效液相色谱 柱前衍生 牛磺酸 长竹蛏 

分 类 号：R151.2[医药卫生—营养与食品卫生学]