超高压处理对枯草芽孢杆菌超微结构的影响  

Effect of Ultra-High Pressure Processing(UHPP) on the Microstructure of Bacillus subtilis

作　　者：曾庆梅[1] 谢慧明[1] 潘见[1] 杨毅[1] 徐慧群[2] 黄训端[1] 赵阳楠[1] 

机构地区：[1]农产品生物化工教育部重点实验室(合肥工业大学),安徽合肥230009 [2]安徽省出入境检验检疫局,安徽合肥230001

出　　处：《高压物理学报》2006年第1期83-87,共5页Chinese Journal of High Pressure Physics

基　　金：国家自然科学基金(30571304);安徽省教育厅重点科研项目(2004kj004zd);农产品生物化工教育部重点实验室开放基金(KF2004005)

摘　　要：研究了超高压处理对枯草芽孢杆菌(Bacillus subtilisAS 1.140)超微结构的影响,探讨其营养体以及芽孢的灭活机制。在经过500 MPa、60℃下保温保压20 min超高压处理后,采用透射电子显微镜进行观察,比较处理前后超微结构的变化。观察结果表明:超高压处理后,枯草芽孢杆菌的营养体细胞壁皱缩、出现缺口,胞浆泄漏、结构层次感消失、出现大片透电子区;其芽孢外壳被破坏、出现缺口,芽孢内含物结构紊乱、泄漏、出现部分透电子区;甚至内含物质完全泄漏,出现细胞壁或孢子外壳残留。The effect of ultra-high pressure processing on the microstructure of Bacillus subtilis AS 1.140 was investigated,and the inactivation mechanism of its vegetative form and spore was studied. The change of B. subtilis microstructures were analyzed and compared in terms of the oberation with a Transmission Electron Microscope （TEM） after UHPP （500 MPa, 60℃, 20 min）. Results showed that the vegetative cell wall of B. subtilis was shrinked, breached and leaked out cytoplasm, the layer structure of cytoplasm disappeared, and a large electron transmission area appeared in the cell after compression. The outer coat of its spores was also breached, the layer structure of spores＇ inclusion disordered, the inclusion leaked out from the cell, and a partial electron transmission area appeared after compression.

关 键 词：枯草芽孢杆菌 超高压处理 超微结构 

分 类 号：O521.9[理学—高压高温物理] Q932[理学—物理]