胡芦巴中性杂多糖酶解工艺优化及其产物对青春双歧杆菌增殖作用的研究  

Optimization for the enzyme hydrolysis of fenugreek neutral heteropolysaccharide and the effects of its products on reproduction of Bifidobacterium adolescentis

作　　者：缪月秋[1] 梅秋红[1] 顾龚平[2] 沈奇[1] 陆长梅[1] 吴国荣[1] 

机构地区：[1]南京师范大学生命科学学院,南京210097 [2]南京野生植物综合利用研究院,南京210042

出　　处：《安徽农业大学学报》2006年第2期238-242,共5页Journal of Anhui Agricultural University

基　　金：国家"十五"科技攻关计划项目(2001BA502B03)资助

摘　　要：对胡芦巴中性杂多糖酶解的工艺条件及其产物对青春双歧杆菌增殖作用进行研究。以还原糖得率为参考指标,采用L9(34)正交设计对反应温度、反应时间、酶量的参数进行优选,观察了水解效率不同的两种方案的酶解产物及其中不同分子量的组分对青春双歧杆菌的增殖效应。结果表明,方案5即反应温度50℃、反应时间9 h和酶量6%,最有利于胡芦巴中性杂多糖的酶解,其产物更有利于双歧杆菌增殖,该方案的胡芦巴中性杂多糖酶解产物经Sephadex G-150柱层析,得到两个主要组分,其均分子量分别为4.5×104和7.5×103,并证实分子量较小的多糖酶解片断更有利于青春双歧杆菌的增殖。This paper studied the enzyme hydrolysis conditions of fenugreek neutral heteropolysaccharide and the effects of its products on reproduction of Bifidobacterium adolescentis. The orthogonal test was used to optimize the reaction temperature, reaction time and enzyme content, using the yield of oligosaccharide as an index, the products of two schemes with different hydrolysis efficiency and different molecular weight components, showed significant effects on the growth of B. adolescentis. The results showed that the No. 5 scheme （9 h reaction time, 50 ℃ reaction temperature and 6% enzyme content） was the optimal condition for enzyme hydrolysis of fenugreek neutral heteropolysaccharide and its products had better effects on the growth of B. adolescentis. Molecular weights of two components, which were from the products separated by Sephadex G-150, were 4. 5 × 10^-4 ,7.5 × 10^3 respectively, and the small one had better effects on B. adolescentis growth.

关 键 词：胡芦巴中性杂多糖 酶解 青春双歧杆菌 增殖作用 

分 类 号：Q946.3[生物学—植物学]