重组鼠源角质细胞生长因子的发酵表达优化及纯化  

Improvement of Fermentation Conditions of Recombinant Mouse Keratinocyte Growth Factor and Its Purification

作　　者：边六交[1] 杨晓燕[1] 樊钰虎[1] 陈爱美[1] 

机构地区：[1]西北大学生命科学院基因工程中心,陕西西安710069

出　　处：《中国医药工业杂志》2006年第5期314-317,共4页Chinese Journal of Pharmaceuticals

基　　金：陕西省科技攻关项目(2003K10-G58)

摘　　要：通过正交实验优化了融合型小鼠角质细胞生长因子(rm-KGF)重组大肠杆菌的发酵和诱导表达条件。优化后的发酵培养基为:葡萄糖6g/L,酵母提取物10g/L,NH4Cl1.3g/L,磷酸盐1mol/L,MgSO4·7H2O0.6g/L。诱导表达条件为:以1mmol/LIPTG于菌体密度(A600)为1时诱导5h。在此条件下,分别经CM-SephadexG-50和HeparinSepharoseCL-6B两步纯化,所得融合rm-KGF经HPLC检测纯度约96%,每升发酵液可得约82mg纯化产品。The fermentation and induced expression conditions of recombination fusion mouse keratinocyte growth factor （rm-KGF） from E. coli were investigated by orthogonal experiment. The optimum culture medium was： glucose 6 g/L, yeast extract 10g/L, NH4Cl 1.3g/L, phosphate solution 1mol/L, MgSO4·7H2O 0.6g/L. The optimized induction condition was： induced at A 600 1.0 with 1 mmol/L IPTG for 5h. After separation and purification by CM-Sephadex G-50 column and Heparin Sepharose CL-6B affinity column, 82mg rm-KGF could be obtained from per liter broth, and the purity was about 96% by HPLC.

关 键 词：角质细胞生长因子 发酵 纯化 正交试验 

分 类 号：TQ92[轻工技术与工程—发酵工程]