有机磷农残检测用植物酯酶的研究  

Phytoesterase Study on Detecting the Residues of Organophosphate Pesticides

作　　者：温艳霞[1] 李建科[1] 

机构地区：[1]陕西师范大学食品工程系,陕西西安710062

出　　处：《食品科学》2006年第4期123-126,共4页Food Science

基　　金：国家科技攻关项目(2001BA804A28);陕西省科技攻关项目(2004k01-G7)

摘　　要：本文研究了多种植物种子中植物酯酶的提取方法和总酯酶活力,得出了最佳提取条件。并且研究了不同来源的植物酯酶对有机磷农药的敏感性,确定了黄豆来源酯酶对敌敌畏等五种农药的最低检测限。结果表明,以黄豆来源的植物酯酶的总酯酶活力和敏感性为最好。The extraction methods for optimum conditions and the general esterase activity of phytoesterase were studied. The paper also researched the sensitivity of phytoesterase extracted from different plant seeds on assaying organophosphate pesticides, and the limits of detection concentration（LDC） of five pesticides were determined. Results showed that the general esterase activity and the sensitivity of the phytoesterase obtained from soybean are the best.

关 键 词：植物酯酶 农药残留 水提条件 检测限 

分 类 号：TS207.53[轻工技术与工程—食品科学]