一种真菌DNA提取方法的改进  

AN IMPROVED METHOD FOR EXTRACTING FUNGAL DNA

作　　者：薛淑静[1] 岳田利[1] 关健[1] 袁亚宏[1] 高振鹏[1] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100

出　　处：《食品研究与开发》2006年第4期39-40,共2页Food Research and Development

基　　金：国家科技部西部专项(2001BA901A19);国家"十五"科技攻关项目(2001BA501A5);霍英东基金项目(81065)

摘　　要：根霉属真菌由于气生菌丝特别发达,菌丝容易产生褐色或黑色色素等,用以前文献报道的方法不易提取DNA或提取的DNA容易降解。本文采用改进的氯化苄法,得到的DNA质量好、产量高,可以直接用于后续的基因操作。此方法具有快速、简便、经济的优点,而且需要样品量少,可用于小剂量样品DNA的提取。The mycelia of Rhizopus increases very rapidly. Aerial myeelium greyish brows ,and then colonies produces a diffusing yellow pigment in the medium. DNA isolation from the filamentous fungi is invalid by the classie methods or the loss of DNA can oecur during the isolation step. DNA from the filamentous fungi could be provided by the modified with benzyl chioride. And the quality of the extraction may satisfy the need of molecular analysis. The method is not only easy and rapid , but also effective to obtain DNA from small-scale DNA sample.

关 键 词：真菌 DNA提取 氯化苄 

分 类 号：Q78[生物学—分子生物学] TS201.3[轻工技术与工程—食品科学]