微胶囊化儿茶素对阿霉素诱导的肾病大鼠黄嘌呤氧化酶、羟自由基与脂质过氧化产物丙二醛的影响  

Effect of catechin microcapsulation on xanthe oxidase, hydroxy radical and malonydialdehyde in rat with nephrotic syndrome induced-adriblastine.

作　　者：何小解[1] 易著文[1] 莫双红[1] 党西强[1] 白海涛[1] 

机构地区：[1]长沙中南大学湘雅二医院小儿肾脏病研究室

出　　处：《中华妇幼临床医学杂志（电子版）》2006年第3期130-133,共4页Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)

基　　金：湖南省自然科学基金(02JJXY3020);湖南省教育厅重点项目基金资助(02A015)

摘　　要：目的探讨微胶囊化儿茶素对阿霉素诱导的肾病大鼠黄嘌呤氧化酶(xanthe oxidase, XOD),羟自由基(hydroxy radical,·OH)与脂质过氧化产物丙二醛(malonydialdehyde,MDA)的影响。方法将60只雌性SD大鼠随机分为对照组、肾病组、地塞米松组、维生素E(vitamin E)组、儿茶素组和微胶囊化儿茶素组,共计6组,尾静脉一次性注射阿霉素(5 mg/kg)制备肾病模型。实验第6周末杀鼠取尿、血、肾及肝脏,利用生化法测定血、肾及肝脏XOD,·OH与MDA含量,利用考马斯亮蓝法测定24 h尿蛋白的排泄量。结果微胶囊组大鼠肝脏中XOD,·OH与MDA含量均显著低于儿茶素组、地塞米松组与vitamin E组;在血清与肾脏中XOD,·OH与MDA含量均显著低于儿茶素组、vitamin E组,高于地塞米松组。实验末24 h尿蛋白排泄量,微胶囊组显著低于儿茶素组。结论微胶囊化儿茶素可能是通过抑制XOD活性, 有效清除·OH,抑制MDA达到降低肾病大鼠24 h尿蛋白排泄的目的。Objective To study the effeet of catechin mieroeapsule on xanthe oxidase （XOD）, hydroxy radieal （^.OH ） and malonydialdehyde （MDA） in rats with nephrotie syndrome induced- adriblastine. Methods 60 female SD rats are randomly distributed in control group, nephrotic syndrome group, gloueortcoid group, vitamin E group, cateehin group and catechin microcapsule group. Nephrotic syndrome rats are caused by injecting adriblastine, before rats were killed, we colleet urine,blood , liver tissue and kidney tissue. XOD, ^.OH and MDA concentration were deteeted by bioehemistry assay, 24 hrs urinary protein excretion were detected by Comb＇s bright blueness assay. Results Liver tissue XOD, ^.OH and MDA concentration of rats in mierocapsule group were lower than that of in catechin group, gloucortcoid group and vitamin E group, kidney tissue and serum XOD, ^.OH and MDA concentration of rats in microcapsule group were lower than that of in eateehin group and vitamin E group, were higher than that of in gloucortcoid group. 24 hrs urinary protein excretion of rats in microcapsule group were lower than that of in catechin group in the end of experiment. Conclusion Catechin microcapsule could succeedly reduce 24 hrs urinary protein excretion in rats with nephrotic syndrome induced-adriblastine might be aehieved through restraining XOD, eliminating ^.OH effieieney, restraining MDA.

关 键 词：儿茶素 微胶囊 肾病变综合征 黄嘌呤氧化酶 羟自由基 脂质过氧化产物丙二醛 

分 类 号：R692[医药卫生—泌尿科学]