高效液相色谱法测定焦糖色素中的4-甲基咪唑  

Determination of 4-Methylimdiazole in caramel pingments by high performance liquid chromatography

作　　者：陈洪兴[1] 戴军 陈尚卫 顾正彪 

机构地区：[1]盐城工学院食品科学与工程系,江苏盐城224003 [2]江南大学分析测试中心,江苏无锡214036

出　　处：《中国调味品》2006年第6期53-56,共4页China Condiment

基　　金：江苏省教育厅指导性研究项目(04KJD550210)

摘　　要：建立了高效液相色谱测焦糖色素中4-甲基咪唑的方法。样品经碱性条件下三氯甲烷-无水乙醇萃取和硫酸水溶液反萃取后,用反向色谱柱HypersilBDSC18(4.6mmi.d.×250mm,5um)分离,以体积比为5∶95的甲醇-0.05mol/L磷酸二氢钾缓冲液(含0.005mol/L庚烷磺酸钠,pH3.5)为流动相,在UV215nm波长条件下检测了自制及几种市售焦糖色素中的4-甲基咪唑的含量。并试验了不同流动相pH值和甲醇浓度对4-甲基咪唑分离的影响。本法平均加标回收率为94.7%,检出限为0.7ng。A novel high performance liquid Chromatographic method for determination of 4-Methylimidazole in caramel pigments was established. The sample was extracted by basic chilroform-enthanol and then by acidic aqueous solution. The chromatography separation was performed on a HypersilBDSC18 column （4. 6mm i. d. × 250mm, 5μm） using methanol-0.05mol/Lpotassium dihydrogen hposphate buffer containing 0. 05mol/L heptanesulfonic acid sodium（5/95, v/v;pH3. 5）as the mobile phase and the detection was at wavelength of uUV-215nm. The method was applid for the determination of 4-Methylimidaz in self-producion and some commercial caramel pigments, and the effects of the different pH value and methanol conecentration in the mobile phase on separation of 4-Methylimidazole were observed. The average recover was 94. 7% and the detecion limit was 0. 7ng.

关 键 词：高效液相色谱法 4-甲基咪唑 焦糖色素 

分 类 号：TS207[轻工技术与工程—食品科学]