纤维堆囊菌发酵产生埃博霉素条件的优化  

Optimized Conditions for Producing Epothilone via Fermentation of Sorangium cellulosum

作　　者：罗立新[1] 汪薇[1] 陆一鸣[1] 潘力[1] 萨斯琳[1] 孔俊文[1] 

机构地区：[1]华南理工大学生物科学与工程学院,广东广州510640

出　　处：《华南理工大学学报（自然科学版）》2006年第5期48-52,共5页Journal of South China University of Technology(Natural Science Edition)

基　　金：广东省自然科学基金资助项目(031357)

摘　　要：埃博霉素是纤维堆囊菌产生的一种具有抗肿瘤活性的次级代谢产物.文中研究了纤维堆囊菌DSM11999发酵合成埃博霉素的条件.结果表明:采用对数生长期后期的菌种在25℃进行发酵,将马铃薯淀粉和胰蛋白胨分别作为碳源和氮源,添加少量的无机盐MnC l4,EDTA-Fe-Na,K2HPO4,ZnC l2,CuSO4和生长促进因子丙氨酸,可使埃博霉素的产量提高22.5%.This paper focuses on the production of epothilone, a kind of secondary anti-cancer metabolite fermented by Sorangium cellulosum DSM11999. The results show that, respectively with potato starch and peptone as the carbon source and the nitrogen source, and with the addition of MnCl4, EDTA-Fe-Na, K2HPO4 , ZnCl2, CuSO4 and growth factor alanine, epothilone can he produced via the fermentation of Sorangium cellulosum DSM11999 in the late exponential phase at 25 ℃, with a yield increased by 22.5%.

关 键 词：纤维堆囊菌 发酵 埃博霉素 优化 

分 类 号：TQ925.4[轻工技术与工程—发酵工程] Q939.1[生物学—微生物学]