三个脐橙品种果实主要细胞壁酶动态变化研究  

Studies on Cell Wall Enzymes of Navel Orange (Citrus sinesis) Fruit

作　　者：曾秀丽[1] 张光伦[1] 李春燕[1] 罗楠[2] 胡强[2] 

机构地区：[1]四川农业大学园艺系,四川雅安625014 [2]四川省农业厅,四川成都610000

出　　处：《亚热带植物科学》2006年第2期12-16,共5页Subtropical Plant Science

基　　金：四川省重大科技攻关项目(2001-08-03-02);四川省农业资源区划重点项目(川农[2003]04号)资助

摘　　要：以罗伯逊脐橙、丰脐、纽荷尔脐橙果实为试材,对其果皮和果肉的果胶甲酯酶(PE)、多聚半乳糖醛酸酶(PG)、β-葡萄糖苷酶和纤维素酶(CX)的动态变化进行研究。结果表明,三个品种PE活性均为果肉明显高于果皮,其中纽荷尔和罗脐分别在果实膨大期、转色期和成熟期出现三个峰值;PE活性变幅纽荷尔大于罗脐,丰脐变化较平稳。PG活性果肉略高果皮,且皆在转色期开始快速升高。首次对脐橙果实β-葡萄糖苷酶研究表明,该酶活性微弱,成熟期活性最高,纽荷尔高于丰脐和罗脐;动态变化中果皮和果肉无明显差异。酶活性高峰出现的时间为PE早于PG和β-葡萄糖苷酶;PE和PG是影响脐橙果实发育的主要酶,β-葡萄糖苷酶可能是PE和PG的补充。Navel orange （Citrus sinensis cvs. Robertson, Fengqi and Newhall） were chosen astesting materials in Zhoupo, Jingyan County. The studies of pectinmethylesterase （PE）,polygalacturonase （PG）, β-glycosidase and cellulase in navel orange during development of fruitswere shown as follows. PE, PG and β-glycosidase could be tested at different stages. PE activity ofpulp was greater than that of peel obviously, the changes of PE activities from Newhall andRobertson navel oranges had three peaks; PE activity of Newhall varied greatly than those ofRobertson and Fengqi. PG activity was greater at maturity stage than at young and the expandingstages; PG activity in pulp was greater than in peel. β-glycosidase activity was tested firstly in fruitsand the peak of β- glycosidase activity was at maturity stage. The peak of PE activity appearedearlier than those of PG and β-glycosidase. PE and PE ere the major enzymes effected thedevelopment of navel orange fruits.

关 键 词：脐橙 果实 果胶甲酯酶 多聚半乳糖醛酸酶 Β-葡萄糖苷酶 纤维素酶 

分 类 号：Q55[生物学—生物化学] S666.4[农业科学—果树学]