反相高效液相色谱-光电二极管阵列检测器法测定杜仲叶发酵液中绿原酸的含量  

Determination of Chlorogenic Acid in Ferment Liquor of Eucommia ulmoides Oliv. Leaves by RP-HPLC-PAD

作　　者：方传代[1] 刘传安[1] 邹盛勤[2] 

机构地区：[1]宜春学院理学院,江西宜春336000 [2]宜春学院生物工程研究所,江西省天然药物活性成分研究重点实验室,江西宜春336000

出　　处：《食品科学》2006年第6期168-170,共3页Food Science

基　　金：江西省教育厅重点资助项目(20002012202)

摘　　要：建立反相高效液相色谱(HPLC)法测定杜仲叶发酵液中绿原酸的含量。色谱条柱SymmetrySheildRP18柱(3.9×300mm,5μm),以水:甲醇:乙酸(88:12:1.2,V/V)为流动相,流速1.0ml/min,检测波长326nm,柱温25℃。绿原酸对照品在2.24～11.2μg范围内线性关系良好(r=0.9993)。本方法简便、准确,线性范围宽,可用于含绿原酸的原料及产品的质量控制。A determination method of chlorogenic acid by high performance fiquid chromtograph and photodiode array detector was estabfished. The factors such as： the chromatographic column, SymmetrySheild RP18 （3.9 × 300mm, 5μ m）, the watermethanol-acetic acid （88：12：1.2, V/V） mobile phase with 1.0ml/min flow rate, the detected wavelength （326nm）, and the column temperature（25 ℃） were adopted. The calibration curve of chlorogenic acid was linear under the content of 2.24 - 11.2μg, with the correlation coefficient 0.9993. The method is simple, accurate and of wider linear range. The method can be adapted to control the quality of material and products that contain chlorogenic acid.

关 键 词：反相高效液相色谱法 杜仲叶 发酵液 绿原酸 

分 类 号：O657.72[理学—分析化学]