利用细胞质导入法选育嗜杀啤酒酵母  

The Breeding of Killer Beer Yeast by the Use of the Cytoduction Technique

作　　者：杜连祥[1] 王昌禄[1] 鲁梅芳 

机构地区：[1]天津轻工业学院,天津300222

出　　处：《生物工程学报》1996年第1期71-77,共7页Chinese Journal of Biotechnology

摘　　要：利用细胞质导入(Cytoduction)法中的核融合缺陷细胞融合技术,在对酿酒酵母(Saccharomyces cerevisiae)D518菌株不做任何遗传标记,将嗜杀酵母5045菌株的嗜杀质粒转移到受体菌D518中,获得了具有两亲株优良性状的融合子KD102菌株。对融合子分析表明:融合子遗传性状稳定,不仅含有供体菌5045的嗜杀质粒,而且受体菌D518的核基因被原封不动地保留下来,为异质体细胞(Heteroplasmon)。将融合子KD102菌株用于小型、中型及生产性酿酒试验,结果表明,具有与亲株D518同样的酿造特性。在发酵过程中,能抑制野生酵母污染,净化发酵体系。对于保证啤酒纯种酿造及提高成品酒的生物稳定性具有明显效果。On condition that don't make any genetic marks for receptor Saccharomyces cermisae D518, killer plasmid of donor strain-killer yeast 5045 was introduced into it by use of the cytoduction technique and one stable fusint in genetics KD102 which to contain the good characters of both parents was obtained. Analyzing the results showed that KD102 was a heteroplasmon which contained the killer plasmid of donor strain 5045 and the nuclear gene of receptor D518. The KD102 slrain had been applied in laboratory scale, medium-size and large scale beer brewing the results indicated that it can inhibit the contamination of wild yeast during the period oi fermentation, purify the fermenting system and improve the biochemical stability of beer.

关 键 词：嗜杀酵母 酿酒酵母 细胞质导入 育种 

分 类 号：TS261.11[轻工技术与工程—发酵工程]