利用纤维二糖的酵母工程菌构建  

Construction of Recombinant Yeast Strain Using Cellobiose as Sole Carbon Source

作　　者：洪剑辉[1] 张梁[1] 石贵阳[1] 王正祥[2] 章克昌[1] 

机构地区：[1]江南大学生物工程学院生物资源研究室,江苏无锡214036 [2]江南大学工业生物技术教育部重点实验室,江苏无锡214036

出　　处：《应用与环境生物学报》2006年第3期391-394,共4页Chinese Journal of Applied and Environmental Biology

基　　金：国家科技部"十五"攻关项目(2001BA501A01)资助~~

摘　　要：以扣囊复膜孢酵母染色体DNA为模板,通过PCR方法扩增到其β-葡萄糖苷酶基因bgl1,经EcoRⅠ、BamHⅠ双酶切并和经相同酶切的穿梭表达载体pYX212连接,构建了重组质粒pYX-sbgl,转化酿酒酵母W 303-1A,bgl1基因获得了活性表达,β-葡萄糖苷酶活力为0.504 IU/mL.转化子能以纤维二糖为唯一碳源生长,并能应用于同时糖化和发酵纤维素底物生产乙醇,使乙醇产量较宿主酵母有了一定的提高.Gene bgll encoding β-glucosidase was cloned from Saccharomycopsis fibuligera. The bgll gene was inserted into shuttling expression vector pYX212 resulting in recombinant plasmid pYX-sbgl. Then, the recombinant plasmid was transformed into Saccharomyces cerevisiae W303-1A and gene bgll was actively expressed in the host strain. The activity of β-glucosidase of the recombinant yeast cell-free extract was 0.504 （IU/mL）. The recombinant yeast strain could grow well on cellobiose as a sole carbon source and could be used for ethanol production through simultaneous saccharification and fermentation （SSF） of cellulose substrate. Fig 6, Tab 1, Ref 14

关 键 词：扣囊复膜孢酵母 Β-葡萄糖苷酶 纤维二糖 同时糖化和发酵 

分 类 号：TQ920[轻工技术与工程—发酵工程]