青霉单宁酶高活性菌株的诱变选育  

Mutation Breeding of High Tannase Activity Strains from Penicillum

作　　者：刘小兰[1] 杨顺楷[2] 王忠彦[1] 

机构地区：[1]四川大学生命科学学院,四川成都610064 [2]中国科学院成都生物研究所,四川成都610041

出　　处：《微生物学杂志》2006年第3期108-110,共3页Journal of Microbiology

摘　　要：利用塔拉单宁诱导丝状真菌产生单宁酶的原理,通过富集培养,从天然源分离得到30株具有较高单宁酶活性的青霉菌;经二级发酵程序,对这30株菌进行了生物转化复筛实验,选择出能水解塔拉单宁,且生物催化活性较高的青霉野生株Penicilliumsp.No.23,对No.23进行经紫外诱变处理,诱变株经筛选,最后得到1株具有稳定遗传性的单宁酶高活性菌株,其单宁酶活性比出发菌株提高了35%。30 strains of Penicillium with higher tannase activity were isolated from natural resource by using enrichment-culture technique on the principle that tannase can be induced by tara tannin. By using two-stage fermentation procedure in the screening studies of bioconversion of tara tannin into gallic acid and quinic acid, Penicillium No. 23 which has the capability of hydrolyzing tara tannin and the high biological catalyzing activity was selected from these 30 strains. Penicillium No. 23 was then mutated by UV treatment. Finally, one strain which had high tannase ac- tivity and stable genetic property was acquired from mutants by means of screening. Its tannase activity has been raised by 35 %.

关 键 词：青霉 单宁酶 紫外诱变 塔拉 没食子酸 奎尼酸 

分 类 号：Q949.327.1[生物学—植物学]