一株啤酒污染菌的分离鉴定及快速检测方法  

Isolation,Identification and Rapid Detection of a Beer Spoilage Bacterium

作　　者：田小群[1] 周世宁[1] 

机构地区：[1]中山大学生命科学学院,广东广州510275

出　　处：《中山大学学报（自然科学版）》2006年第4期70-74,共5页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家自然科学基金资助项目(30370030)

摘　　要：在成品瓶装啤酒中分离得到l株有害的污染菌。经表型性状分析、生理生化反应、G＋C含量测定以及16SrDNA序列分析，鉴定为Lactobacillus acetotolerans。建立了1种新的快速鉴定啤酒有害菌的PCR方法，这种方法基于抗酒花基因horA的部分序列。用传统的检测方法检测有害菌需要8-10d，而用新的PCR方法仅需24～32h。A new beer spoilage bacterium was isolated in a bottle of product beer. It was identified as Lactobacillus acetotolerans by type description , biochemical test , G ＋ C% determination and 16S rDNA sequence analysis. A new method for rapid detecting beer-spoilage Lactobacillus by PCR was developed. This method was based on the sequence of a hop-resistance gene horA. The time for the beer-spoilage Lactobacillus acetotolerans detection was only 24 -32 h by the PCR method.

关 键 词：啤酒有害菌 快速鉴定 PCR LACTOBACILLUS acetotolerans 

分 类 号：Q93[生物学—微生物学]