酶法制取比目鱼皮胶原蛋白寡肽  

ENZYMATIC PRODUCTION OF FLATFISH SKIN COLLAGEN OLIGOPEPTIDE

作　　者：韩凤杰[1] 赵征[1] 

机构地区：[1]天津科技大学食品科学与生物技术学院,天津300457

出　　处：《食品研究与开发》2006年第8期104-107,共4页Food Research and Development

摘　　要：选用比目鱼皮作为一种新型的胶原蛋白资源,首先对其进行预处理,然后用2709碱性蛋白酶水解。利用单因素试验和响应面分析优化2709碱性蛋白酶水解比目鱼皮的最佳工艺条件为:pH10;底物质量浓度70g/L;温度55℃;加酶量4%;水解2h;最大水解度为22.36%。然后对水解产物进行后处理,得到了一套完整的生产鱼皮胶原蛋白寡肽的工艺。用飞行时间质谱仪测定所得水解产物的分子质量分布范围,结果表明其分子质量主要集中在0.6kDa～1.8kDa之间。Flatfish （Paralichthys olivaceus ） skin is used as a new kind of collagen resource, firstly retreat the raw material, then 2709 alkaline protease was used to hydrolyze. The single factor experiment and Response Surface Methodology were used to optimize the technical parameters, the optimal technical parameters were obtained as following： pH 10, concentration of substrate 70 g/L, ration of 2709 alkaline protease 4 %, temperature 55 ℃, and the maximum hydrolysis degree was 22.36 %.Then the hydrolysates was treated, we gained a suit of whole technics to produce fishskin collagen oligopeptide. MALDI-TOF-MS was used to determine the molecular weight distribution of hydrolysates, the results indicated that the molecular weight of the majority was between 0.6 kD and 1.8 kD.

关 键 词：比目鱼皮 胶原蛋白 蛋白酶 水解 寡肽 

分 类 号：TS254[轻工技术与工程—水产品加工及贮藏工程]