反相高效液相色谱法同时测定保健品中的四种类胡萝卜素  

Simultaneous Determination of Four Carotenoids in Health Products by Reversed-Phase High Performance Liquid Chromatography

作　　者：李赫[1] 陈敏[1] 朱蕾[1] 刘丽娟[1] 王静钰[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《色谱》2006年第5期475-478,共4页Chinese Journal of Chromatography

基　　金：国家科技攻关计划资助项目(No.2001BA804A21)

摘　　要：建立了不同剂型保健品中类胡萝h素的提取方法和同时测定保健品中4种类胡萝h素含量的高效液相色谱分析方法。采用Diamonsil^TM C18色谱柱（250mm×4．6mmi．d．，5μm），以乙腈和乙酸乙酯为流动相进行梯度洗脱，洗脱程序：先以100％乙腈洗脱15min，然后乙酸乙酯的含量在10min内从0增加到100％，再以100％乙腈洗脱5min；流速1mL／min；采用二极管阵列检测器检测，检测波长为450nm。以外标法定量，番茄红素、叶黄素、玉米黄素、β-胡萝h素4种组分的线性关系良好，相关系数为0．9994～0．9997，检测限为0．4～0．5mg／L。片剂样品、粉状样品、油状样品的加标回收率分别为95．3％～98．7％，93．7％～98．8％，97．1％～99．2％，相对标准偏差（RSD）分别为0．89％～2．0％，0．89％-2．8％，0．42％1．2％。该法简便、快速、准确，是保健品中多种类胡萝卜素定量测定的可靠方法之一。A method was established for the determination of carotenoids （lutein, zeaxanthin, lycopene, β-carotene） in health products by reversed-phase high performance liquid chromatography （RP-HPLC）. The extraction methods of carotenoids from different types of samples were also established. Separation and quantification was achieved by using a Diamonsil^TM C18 column （250 mm × 4.6 mm i. d. , 5 μm）, and a mobile phase of acetonitrile and ethyl acetate were used with the following gradient elution： 100% acetonitrile at the first 15 min, then ethyl acetate increased to 100% in the next 10 min, at last 100% acetonitrile for 5 min with a flow rate of 1 mL/min, temperature of 25 ℃ and monitored with a photodiode array detector at 450 nm. The external standard calibration curves were used for quantification. There were good linear correlations for the four carotenoids. The correlation coefficients were between 0.999 4 and 0.999 7. The detection limits were 0.4 -0.5 mg/L. The recoveries of four carotenoids in tablet samples were 95.3% - 98.7% with the relative standard deviations （RSDs） of 0.89% - 2.0%. The recoveries of four carotenoids in powder samples were 93.7% - 98.8% with RSDs of 0.89% -2.8%. The recoveries of four carotenoids in oil samples were 97. 1% -99.2% with RSDs of 0.42% - 1.2%. This method is simple, fast, accurate and convenient. It can be used as one of the reliable means for quantitative determination of carotenoid in health products.

关 键 词：反相高效液相色谱 类胡萝卜素 番茄红素 叶黄素 玉米黄素 Β-胡萝卜素 保健品 

分 类 号：O658[理学—分析化学]